Infection of tumor cells by every attenuated Lm construct resulted in cell death by activation of NADPH oxidase and elevated levels of cytosolic reactive oxidative species (ROS)., 1995a,b)., 2004; Wallecha et al., 2009; Ishizaki et al., 2010)., 2009; Quispe-Tintaya et al., current work has confirmed the tumor-homing properties of Lm (Yu et al., 2013)., 2009). Devoid of prfA complementation, this mutant Lm can’t escape the phagosome and is destroyed by macrophages and neutrophils.
A far more current study by precisely the same laboratory documented a similar tumor-homing ability of an attenuated Lm vector that expresses truncated LLO from an episomal plasmid (Listeriaat ) to pancreatic tumors in mice (Quispe-Tintaya et al. Another strategy is primarily based around the in vitro and in vivo complementation of D-alanine racemase in each E. Chromosomal integration methods can use either a phage-based technique, having a site-specific integrase to integrate at al., 2013b)
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